Comparative Results of Survival of Vitrified Biopsied Goat Embryos and Mouse Morulae

Authors : Aqin CHEN, Rui ZHANG, Songdong YU

Pages : 93-97

View : 13 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The purpose of this study was to find an efficient cryoprotectant and a suitable procedure for vitrification of mouse morulae. The method found with mouse morulae was then applied to the cryopreservation of biopsied goat embryos. Mouse morulae were exposed, in 1- or 2-step, to the ethylene glycol insert ignore into journalissuearticles values(EG);-based solutions EFS30 or EDFS30, which contained 30% EG and 15% EG plus 15% dimethylsulphoxide insert ignore into journalissuearticles values(DMSO);, respectively, in phosphate buffer saline insert ignore into journalissuearticles values(PBS); containing 30% insert ignore into journalissuearticles values(w/v); Ficoll and 0.5 M sucrose, cooled in liquid nitrogen and warmed up rapidly. After warming, the rate of the hatched blastocyst formation in mouse morulae vitrified with EFS30 insert ignore into journalissuearticles values(54.0%); by the 2-step method was significantly higher compared to those vitrified with EFS30 insert ignore into journalissuearticles values(30.4%); and EDFS30 insert ignore into journalissuearticles values(27.7%); by the 1-step method insert ignore into journalissuearticles values(P < 0.05);. According to those results, biopsied goat embryos were vitrified in EFS30 using the 2-step method. The results showed that the developmental blastocyst rate of the post-thawed embryos was 47.1%. It may be concluded that goat embryos were vitrified safely in an ethylene glycol-based solution insert ignore into journalissuearticles values(EFS30); by the 2-step method.
Keywords : Mouse morulae, biopsied goat embryos, vitrification, cryopreservation