Determination of Clostridium perfringens Toxin-Types in Sheep with Suspected Enterotoxemia in Kars Province, Turkey

Authors : Halil İbrahim GÖKCE, Oktay GENÇ, Mahmut SÖZMEN, Gürbüz GÖKÇE

Pages : 355-360

View : 26 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in Kars province. For this purpose, 260 dead sheep with suspected enterotoxemia and 35 clinically healthy sheep insert ignore into journalissuearticles values(controls); of different ages and breeds were used. Intestinal contents of each sheep were collected and used to determine alpha, beta, and epsilon toxins of C. perfringens using enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA); and the latex agglutination test insert ignore into journalissuearticles values(LAT);. Based on ELISA and LAT, 220 insert ignore into journalissuearticles values(84.61%); and 152 insert ignore into journalissuearticles values(58.46%); sheep, respectively, were positive for C. perfringens toxins. In contrast, 40 insert ignore into journalissuearticles values(15.38%); sheep were toxin-negative based on ELISA and 108 insert ignore into journalissuearticles values(41.53%); were toxin-negative based on LAT. With ELISA, 104 insert ignore into journalissuearticles values(47.27%);, 20 insert ignore into journalissuearticles values(9.09%);, 10 insert ignore into journalissuearticles values(4.54%);, and 86 insert ignore into journalissuearticles values(39.07%); toxin-positive samples were identified as C. perfringens type A, B, C, and D, respectively, whereas with LAT, 79 insert ignore into journalissuearticles values(51.97%);, 15 insert ignore into journalissuearticles values(9.86%);, 7 insert ignore into journalissuearticles values(4.60%);, and 51 insert ignore into journalissuearticles values(33.55%); toxin-positive samples were identified as type A, B, C, and D, respectively. C. perfringens type A and type D were the dominant types detected by both tests in sheep with suspected enterotoxemia, in Kars province. It is concluded that enterotoxemia causes considerable economic loss to the sheep industry in Kars province, where 300,000 sheep are present. Therefore, it is recommended that a proper vaccination schedule against enterotoxemia be implemented for flocks in Kars province. These vaccines should provide adequate protective immunity against both C. perfringens type A and type D.
Keywords : Sheep, Clostridium perfringens, alpha toxin, beta toxin, epsilon toxin, enterotoxaemia, enzyme linked immunosorbent assay ELISA, latex agglutination test LAT,