Detection of Listeria monocytogenes by using PCR in Helix pomatia

Authors : Şükrü KIRKAN, Ergün Ö GÖKSOY, Osman KAYA

Pages : 375-380

View : 19 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The detection of Listeria monocytogenes in Helix pomatia, both raw and cooked is presented here. Polymerase chain reaction insert ignore into journalissuearticles values(PCR); is a rapid procedure with both sensitivity and specificity for quick detection and identification of Listeria monocytogenes from raw and cooked Helix pomatia. A total of 30 bags insert ignore into journalissuearticles values(10 g each); of H. pomatia samples were investigated for the Listeria monocytogenes inlB gene with the PCR method. PCR amplification products demonstrated that 18 of the 30 samples showed positive reactions to Listeria monocytogenes in the PCR. All PCR positive samples showed specific amplification of the 343 bp fragment for Listeria monocytogenes.
Keywords : Listeria monocytogenes, Helix pomatia, PCR