Use of Indirect Excretory/Secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the Diagnosis of Natural Fasciola hepatica Infection in Eosinophilic and Non-Eosinophilic Cattle from Eastern Turkey

Authors : Sami ŞİMŞEK, Ergün KÖROĞLU, Armağan Erdem ÜTÜK, Kürşat ALTAY

Pages : 411-415

View : 22 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to investigate fasciolosis in a group of eosinophilic and non-eosinophilic cattle in eastern Turkey. All the cattle were examined for antibodies against F. hepatica by modified indirect excretory/secretory enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ES-ELISA);. Of the 1200 cattle tested, 164 insert ignore into journalissuearticles values(13.6%); were found to be eosinophilic by absolute eosinophil counts. Out of the 164 eosinophilic cattle, 121 insert ignore into journalissuearticles values(73.7%); were found to be positive for fasciolosis by indirect ES-ELISA, while the rate was only 35% among the non-eosinophilic group insert ignore into journalissuearticles values(P < 0.001);. Our data indicate that parasitic infections should be considered an important cause of eosinophilia, particularly in fasciolosis, and the indirect ES-ELISA is useful as a screening test to examine anti-F. hepatica antibodies for the diagnosis of fasciolosis in cattle.
Keywords : Fasciola hepatica, eosinophilia, ES ELISA, cattle, Turkey