Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels

Authors : Ergün Ö GÖKSOY, Şükrü KIRKAN, Osman KAYA

Pages : 229-234

View : 17 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.
Keywords : Listeria monocytogenes, stuffed mussel, conventional methods, PCR