The Red Blood Cell Membrane Proteins in Rabbits with Experimental Ketosis

Authors : Sefa ÇELİK

Pages : 151-155

View : 18 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study investigated the effects of experimentally induced ketosis on erythrocyte membrane proteins in rabbits by sodium dodecyl sulfate/polyacrylamide gel electrophoresis insert ignore into journalissuearticles values(SDS-PAGE);. The animals were allocated to 2 groups; ketosis and control. The experiment was lasted 13 days. Food was withheld for 5 days in the ketosis group, and then the animals were fed for 8 days. The control group was fed standard rabbit rations. Erythrocyte membrane proteins were isolated from the blood, and analyzed by SDS-PAGE using 10% insert ignore into journalissuearticles values(w/v); acrylamide monomer. Erythrocyte membrane proteins separated on the polyacrylamide gel were quantified by densitometry. Band 3 and actin on day 1, and spectrin on day 5 were significantly increased insert ignore into journalissuearticles values(P < 0.05);, while glyceraldehyde-3-phosphate dehydrogenase insert ignore into journalissuearticles values(G3PD, E.C. 1.2.1.12); was significantly decreased insert ignore into journalissuearticles values(P < 0.01); on day 5 in the ketosis group. On the other hand, ankyrin increased insert ignore into journalissuearticles values(P < 0.05); when compared to the controls on day 6 of the trial. The results show that ketosis may lead to quantitative changes in erythrocyte membrane proteins including band 3, actin, spectrin, G3PD and ankyrin.
Keywords : Rabbit, ketosis, erythrocyte membrane protein, SDS PAGE