Spawning Induction in the Common Carp (Cyprinus carpio) Using Different Combinations of [D-Ser(tBu)6,Pro9-Net]-LHRHa and Metoclopramide

Authors : Muhammed ARABACI

Pages : 1051-1056

View : 19 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The effects of a single administration of [D-Serinsert ignore into journalissuearticles values(tBu);6,Pro9-NEt]-LHRHa at high dose alone insert ignore into journalissuearticles values(50 µg/kg);, and at constant dose insert ignore into journalissuearticles values(20 µg/kg); combined with different doses of the dopamine receptor antagonist Metoclopramide insert ignore into journalissuearticles values(5, 10, 20, 40 mg/kg); for the induction of spawning in common carp broodstocks were determined under local hatchery conditions and compared with classic carp pituitary extract insert ignore into journalissuearticles values(CPE, double injection); application insert ignore into journalissuearticles values(water temperature 24 ºC);. Fish injected with physiological saline insert ignore into journalissuearticles values(0.7% NaCl); were used as a control group. No spawning was observed in the control group or in the groups given LHRH-a combined with low insert ignore into journalissuearticles values(5, 10 mg/kg); doses of MET. The spawning ratio was low in the group given a high dose of LHRH-a alone insert ignore into journalissuearticles values(50 µg/kg);, while it was high in the groups given LHRH-a combined with high insert ignore into journalissuearticles values(20, 40 mg/kg); doses of MET, and in the group injected with CPE. Latency period in the LHRH-a+MET groups was approximately half of that in the CPE group insert ignore into journalissuearticles values(14-16 and 24-26 h, respectively);. There were no differences between spawned groups with respect to the spawning index of broodstocks, or the fertilization/hatching rate of eggs. It was concluded that spawning can be induced successfully in common carp broodstocks with 20 µg/kg LHRH-a+20 mg/kg MET, in a single injection, in a shorter latency period and without a negative effect on egg quality, compared to CPE treatment. This combination may be useful for more effective broodstock and hatchery management in common carp culture.
Keywords : Induced spawning, LHRHa, metoclopramide, Cyprinus carpio