Evaluation of the In Situ Degradation Kinetics of Some Protein Sources Commonly Used in Ruminant Feeding

Authors : Suphi DENİZ, M Akif KARSLI, Hüseyin NURSOY

Pages : 1079-1086

View : 26 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of the study was to evaluate the in situ degradation kinetics of some commonly used protein sources insert ignore into journalissuearticles values(sunflower meal, cottonseed meal, and soybean meal);. Three ruminally fistulated rams were utilized in the study. They were fed ground alfalfa and a mixture of concentrate insert ignore into journalissuearticles values(60% alfalfa and 40% mixture); at maintenance level. The Dacron bags used in the study had a 45 µ2 pore size and internal dimensions of 15 x 7 cm. Feed samples were ground to pass through a 2 mm screen. Samples were incubated in the rumen of rams for 0, 2, 4, 8, 12, 24 and 48 h. Each feed sample was incubated in the rumen of 3 rams in duplicate for each incubation time. Approximately 3.5 g of samples were placed in each Dacron bag. Concentrations of organic matter were 93.16%, 94.43% and 93.40% insert ignore into journalissuearticles values(P < 0.05);; ether extract 1.70%, 7.12% and 2.79% insert ignore into journalissuearticles values(P < 0.05);; crude fiber 21.64%, 19.81% and 6.77% insert ignore into journalissuearticles values(P < 0.05);; crude protein 32.03%, 30.07% and 50.02% insert ignore into journalissuearticles values(P < 0.05);; and ADIN-N 11.57%, 11.00% and 10.45% insert ignore into journalissuearticles values(P < 0.05); for sunflower meal insert ignore into journalissuearticles values(SFM);, cottonseed meal insert ignore into journalissuearticles values(CSM); and soybean meal insert ignore into journalissuearticles values(SBM);, respectively. Ruminal DM degradability of samples was highest with SBM for all incubation times followed by SFM and CSM in that order insert ignore into journalissuearticles values(P < 0.05);. While crude protein degradabilities of SFM were significantly higher insert ignore into journalissuearticles values(P < 0.05); than those of CSM and SBM up to 12 h incubation, they were significantly higher insert ignore into journalissuearticles values(P < 0.05); than those of CSM but similar to those of SBM at 24 and 48 h incubation times. Crude protein degradabilities were 55.72%, 41.08% and 66.06% after 8 h incubation for SFM, CSM and SBM, respectively insert ignore into journalissuearticles values(P < 0.05);. Concentrations of water soluble protein were 45.93%, 37.65% and 35.79% insert ignore into journalissuearticles values(P < 0.05);; potentially degradable protein 44.21% , 29.48% and 55.72% insert ignore into journalissuearticles values(P < 0.05);; non-degradable protein 9.86%, 32.87% and 8.49% insert ignore into journalissuearticles values(P < 0.05);; k, h-1 were 0.173, 0.066 and 0.114 insert ignore into journalissuearticles values(P < 0.05);; percentages of escape protein were 18.41%, 42.50% and 33.55% insert ignore into journalissuearticles values(P < 0.05);; and escape protein 57.90, 130.30 and 167.80 g/kg insert ignore into journalissuearticles values(P < 0.05); for SFM, CSM and SBM, respectively. In conclusion, the concentration of water soluble protein was highest with SFM, potentially degradable protein content was highest with SBM, and non-degradable protein concentration was highest with CSM among the 3 protein sources. However, the highest escape protein concentration was obtained insert ignore into journalissuearticles values(167.8 g/kg); with SBM.
Keywords : Sunflower meal, cottonseed meal, soybean meal, degradation kinetics, escape protein