Isolation of Bacterial Agents from the Lungs of Cattle with Pneumonia and Detection of Pasteurella Spp. by Polymerase Chain Reaction

Authors : Ayşe KILIÇ, Adile MUZ

Pages : 217-223

View : 18 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Lungs from 8222 cattle slaughtered at an abattoir in Elazığ were examined macroscopically, and pneumonia was detected in 500 insert ignore into journalissuearticles values(6.1%); lungs. These samples were inoculated onto blood agar supplemented with 7% sheep blood for isolation of bacterial agents. A polymerase chain reaction insert ignore into journalissuearticles values(PCR); based upon the use of species-specific primers was carried out on DNA samples extracted from suspected Pasteurella spp. isolates. In addition, a mouse inoculation test was carried out on suspected Pasteurella multocida isolates. A total of 311 insert ignore into journalissuearticles values(62.2%); bacterial agents were isolated from the lung samples and were identified as 7.2% Staphylococcus aureus, 6% Pasteurella multocida, 6% Corynebacterium spp., 5.8% yeast, 3.8% Streptococcus spp, 3.6% Staphylococcus epidermidis, 2.6% Escherichia coli, 2.2% Bacillus spp., 2% Pseudomonas spp., 1.8% Mannheimia haemolytica, 1.6% Actinobacillus spp., 1.2% Klebsiella spp., 0.2% Moraxella spp., 0.2% Proteus spp., and 18% mixed bacteria.Twenty-four insert ignore into journalissuearticles values(80%); of the Pasteurella multocida isolates were positive in the mouse inoculation test in cattle. All P. multocida and M. haemolytica strains that were positive by culture were also found to be positive by PCR. However, toxigenic Pasteurella multocida was not detected in any isolates by PCR using primers derived from the toxA gene.
Keywords : Pneumonia, cattle, Pasteurella multocida, Mannheimia haemolytica, polymerase chain reaction PCR, culture