Studies on the in vitro Maturation of Cat Oocytes

Authors : Mithat EVECEN, Hatem ATALLA, Sema BİRLER, Alper BARAN, Kemal AK

Pages : 1409-1412

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of the present study was to investigate the effects of gonadotrophin and Bovine Serum Albumin insert ignore into journalissuearticles values(BSA); supplemented Synthetic Oviduct Fluid insert ignore into journalissuearticles values(SOF); and Ham`s F-10 media on the in vitro maturation of cat oocytes. Oocytes collected from spayed stray queens served as the material of the study. The ovaries were brought to the laboratory within 2 h in PBS solution at 38 °C. Recovered oocytes were divided into two groups insert ignore into journalissuearticles values(Group 1: SOF + 0,4% BSA + 10 µg/ml FSH + 10 µg/ml LH, group 2: Ham`s F-10 + 0,4% BSA + 10 µg/ml FSH + 10 µg/ml LH); and left for maturation in an incubator at 38.5 °C for 48 h under atmosphere containing 5% O2, 5% CO2 and 90 % N2 and almost 100% humidity. Oocytes were then fixed and stained. The maturation status of the oocytes was evaluated under a phase-contrast microscope at x400 magnification. Data were compared by using Student`s t-test. Group 1 had 209 oocytes and group 2 had 207: a total of 416. In group 1 54.06% insert ignore into journalissuearticles values(113/209); and in group 2 29.00% insert ignore into journalissuearticles values(60/207); of the oocytes reached the M II stage. The difference between these values was statistically significant. At the end of the study, it was concluded that SOF medium was superior to Ham`s F-10 medium for the in vitro maturation of cat oocytes. These results also showed that a satisfactory background for in vitro fertilization studies of cat oocytes has been established.
Keywords : Cat, oocyte, in vitro maturation, medium