Development of an Enzyme Immunoassay for the Determination of Testosterone

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Turkish Journal of Veterinary and Animal Sciences
Volume:27 Issue:1
Development of an Enzyme Immunoassay for the Determination of Testosterone

Development of an Enzyme Immunoassay for the Determination of Testosterone

Authors : Bülent GÜVEN

Pages : 45-50

View : 19 | Download : 14

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography insert ignore into journalissuearticles values(sephadex G-25);. Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be
Keywords : Testosterone, enzyme immunoassay

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