Detection of Infectious Bronchitis Virus in Tracheal Organ Cultures Using the Immunoperoxidase Technique

Authors : Ayşin ŞEN

Pages : 1381-1388

View : 19 | Download : 11

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The applicability of the immunoperoxidase insert ignore into journalissuearticles values(IP); technique in unfixed-fresh tracheal organ cultures insert ignore into journalissuearticles values(TOCs); was investigated for rapid detection of infectious bronchitis virus insert ignore into journalissuearticles values(IBV);. For this purpose, TOCs prepared from one-day-old SPF chicks were inoculated with IBV M41 strain in serial dilutions. IBV antigen detected by IP technique in TOCs 18 h postinoculation. IP staining was observed before siliostasis in all dilutions. The IP technique was also applied to detect IBV antigen in formalin-fixed, paraffin-embedded sections of TOCs inoculated with IBV M41 strain. Viral antigen was also detected in these sections. Histopathological examinations of IBV M41 infected tracheal rings showed loss of cilia, flattening of lamina epithelialis, and rounding and desquamation of epithelial cells.
Keywords : Infectious bronchitis virus, Tracheal organ cultures, Immunoperoxidase