Use of Milk Progesterone Assay and Rectal Palpation to Monitor Postpartum Anoestrus and the Effect of PRID-PMSG Treatment in Murrah Buffaloes

Authors : Mehmet UÇAR

Pages : 1389-1393

View : 21 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Postpartum anoestrus is widely known to cause infertility in buffaloes. This study was conducted to determine the problem of postpartum anoestrus using rectal palpation and milk progesterone level in 8 Murrah buffaloes calved during late spring or early summer in the Kocatepe Agricultural Research Institute, Afyon, Turkey. In addition, the pregnancy rates of experimental animals were investigated using a combined therapy of a progesterone releasing intravaginal device insert ignore into journalissuearticles values(PRID); and pregnant mare serum gonadotropin insert ignore into journalissuearticles values(PMSG);. The ovaries of each buffalo were examined by rectal palpation once a week and the milk progesterone level was detected twice a week using enzyme immunoassay insert ignore into journalissuearticles values(EIA); during an oestrus cycle and the week following insert ignore into journalissuearticles values(for a period of a month);. At the end of this period, the PRID was inserted and kept in the vagina for 12 days. Upon removal of the PRID, PMSG insert ignore into journalissuearticles values(750 IU); was administered intramuscularly. Prior to the PRID-PMSG treatment, there were no ovarian activities in any animals and their milk progesterone levels were between 0.12 and 0.94 ng/ml, except for one animal. Approximately 60 h following PMSG administration, all buffaloes insert ignore into journalissuearticles values(100%); showed signs of clinical oestrus. Gestation occurred in all buffaloes with a service per conception rate of 2.13. The results of the present study suggested that milk progesterone level is a useful tool to diagnose postpartum anoestrus and PRID-PMSG treatment induces ovaries, cures anoestrus, and facilitates gestation in Murrah buffaloes.
Keywords : Murrah buffalo, anoestrus, progesterone, PRID, PMSG