Membrane Specific Carbonic Anhydrase (CA-IV) Expression in Bovine Lung: The Effects of Alcoholic and Non-Alcoholic Drinks

Authors : Nazan DEMİR, Hayrunnisa NADAROĞLU

Pages : 227-233

View : 18 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Carbonic anhydrase insert ignore into journalissuearticles values(CA); insert ignore into journalissuearticles values(carbonate hydrolyase: E. C. 4.2.1.1); from bovine lung was purified by a new method and characterized. The purification level was 4306-fold. The optimum temperature for maximum enzyme activity was 37.5°C. The optimum pH was 7.4, varying between 3.5 and 10.0. SDS-polyacryamide gel electrophoresis insert ignore into journalissuearticles values(3-10% discontinuous SDS-PAGE); showed two distinct bands for CA-IV. The molecular weights of the enzymes were found to be approximately 54.000 and 29.000, respectively. Vmax and KM values were calculated with p-nitrophenyl acetate as substrate insert ignore into journalissuearticles values(166.7 µmol/L*min, 5.59*10-2 M, respectively);. Changes in enzyme activity were determined in the presence of some chemicals: sulfanilamide, NaN3, KSCN, glucose, nicotine, nicotinamide, caffeine, CuSO4 and MnCl2; and some drinks: brandy, vodka, raki, whisky, cognac, beer, martini, apple liqueur, red wine, Pepsi, Yedigün, 7UP, Vimto and Coca-Cola insert ignore into journalissuearticles values(carbonated and non-carbonated);.
Keywords : Carbonic anhydrase IV, Alcohol, Nicotine, Nicotinamide, Caffeine