Diagnosis of Lymphoid Leukosis (LL) by ELISA (Enzyme-Linked Immunosorbent Assay) and Marek`s Disease (MD) by IF (Immunofluorescence) Test in Chickens

Authors : Selda ÖZBİLGİN, Ayşin ŞEN, Mihriban ÜLGEN, K Tayfun ÇARLI

Pages : 839-846

View : 32 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, our aim was to diagnose lymphoid leukosis and Marek`s disease, which are neoplastic diseases of chickens, by ELISA and Indirect Immunofluorescence Test insert ignore into journalissuearticles values(IIF);, respectively. Samples insert ignore into journalissuearticles values(eggs and tissues); were selectively taken from the flocks suspected of having lymphoid leukosis and Marek`s disease. The tissues were macroscopically and microscopically examined. Tissues belonging to some flocks showed typical lesions for lymphoid leukosis and Marek`s disease. Seven broiler breede flocks and three commercial layer flocks were tested for avian leukosis virus insert ignore into journalissuearticles values(ALV); group specific antigen insert ignore into journalissuearticles values(p27); in the albumen. ALV p27 antigen was detected in five out of ten flocks. The rate of ALV p27 antigen positive albumens among the flocks examined ranged from 10 to 42%. The lymphoid leukosis infection rate was found to be 15.06% insert ignore into journalissuearticles values(36/239 eggs);. All the flocks with lymphoid leukosis were broiler breeders and they were 23-33 weeks old. In the diagnosis of Marek`s disease, bone marrow smears were used for the detection of MDV antigen by IIF. Of fifteen flocks examined, twelve were determined to be positive. Fluorescent antigen-positive cells were seen in large numbers and these cells exhibited diffuse cytoplasmic staining with discrete granules. Results showed that smears of bone marrow cells are a suitable test material for a rapid and specific diagnosis of MDV infection in chickens. The chickens infected with MDV were 10-20 weeks old.
Keywords : Lymphoid leukosis, ELISA, Marek`s disease, Immunofluorescence, Chicken