Detection of Antibodies Against Salmonella Enteritidis in Chicken Serum and Egg Yolk with ELISA

Authors : Gülay ALTAY

Pages : 983-988

View : 17 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A total of 473 sera from hens of different age groups and different breeds and a total of 430 eggs were collected from 11 breeders and egg producers in the districts of Adapazarı, Afyon, Ankara and Konya. Lipopolysaccharide insert ignore into journalissuearticles values(LPS);, heat-extract insert ignore into journalissuearticles values(HE); and flagella antigens were tested in the standardizing ELISA technique. Flagella antigen was found useful in the screening of field samples. Of the 48 insert ignore into journalissuearticles values(10.2%); rapid plate agglutination positive sera, 7 were negative in ELISA. One hundred and seventy-three insert ignore into journalissuearticles values(36.6%); of the 473 sera and 76 insert ignore into journalissuearticles values(17.7%); of the 430 egg yolks were found to be positive with ELISA. Forty-one insert ignore into journalissuearticles values(23.7%); of the ELISA positive sera were also positive in the rapid plate agglutination test.
Keywords : ELISA, Salmonella enteritidis, serum, chicken, egg yolk