Comparison of Ovine Spermatozoal Morphological Features After Staining of Fixation and Assessment of Morphological Abnormalities in Dead/Live Spermatozoa

Authors : Yavuz ÖZTÜRKLER

Pages : 675-680

View : 16 | Download : 12

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The objectives of this study were to insert ignore into journalissuearticles values(i); compare the effects of two staining techniques and one fixative solution on the morphological features of ram spermatozoa and insert ignore into journalissuearticles values(ii); evaluate the morphological features of dead/live spermatozoa by using Eosin-Nigrosin staining. The morphological features of raw spermatozoa were evaluated insert ignore into journalissuearticles values(by Phase Contrast microscopy); after staining with Eosin-Nigrosin mixture or Nigrosin insert ignore into journalissuearticles values(alone);, or after fixation with Hancock`s buffered formol saline solution. 42 raw pooled semen samples from 6 Kıvırcık rams were used. The percentages of spermatozoa with abnormal acrosomes and total morphological abnormalities averaged 3.97% and 14.78% in Hancock`s solution, compared with averages of 10.07% and 18.54%; 7.97% and 18.45% for smears stained with Eosin-Nigrosin or Nigrosin insert ignore into journalissuearticles values(alone);, respectively insert ignore into journalissuearticles values(P
Keywords : Ram, spermatozoa, fixation, staining