Effect of CZB Medium on the Two Cell Block of Preimplantation Mouse Emryos

Authors : Haydar BAĞIŞ

Pages : 725-729

View : 17 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This experiment was designed to determine the effects of CZB medium on in vitro culture of murine embryos obtained from FVB/N, CD-1, and CB6F1 strains. Pronuclear stage embryos were recovered from superovulated and mated females 17 h post-hCG. Two-cell stage block, morula and blastocyst stages were used as the end point of the experiments. There is no statistical difference among strains and media for the development to the two-cell stage. However, more FVB/N embryos insert ignore into journalissuearticles values(88.3 and 82.4%); than CD-1 insert ignore into journalissuearticles values(57.4 and 57.1%); and CB6F1 insert ignore into journalissuearticles values(69.8 and 28.9%); reached the 3-8 cell stage in CZB and M16 media, respectively. CB6F1 embryos developed better insert ignore into journalissuearticles values(69.8%); in CZB than in M16 insert ignore into journalissuearticles values(28.9%);. Morula development for FVB/N embryos was superior insert ignore into journalissuearticles values(75.0%); in CZB when compared to M16. Collectively, these data suggest that there are strain differences for in vitro embryo development and the in vitro culture medium has a profound effect on in vitro development.
Keywords : Mouse strains, embryo, 2 cell block, in vitro culture