Comparison of ELISA with Other Serological Tests in the Diagnosis of Ovine Brucellosis

Authors : Hasan ÖNGÖR, Adile MUZ, Burhan ÇETİNKAYA

Pages : 21-26

View : 25 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study,antibodies against Brucella melitensis in aborted sheep were investigated with enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA);, standard tube agglutination test insert ignore into journalissuearticles values(SAT);, rose bengal plate test insert ignore into journalissuearticles values(RBPT);, complement fixation test insert ignore into journalissuearticles values(CFT);, 2-mercaptoethanol microagglutination test insert ignore into journalissuearticles values(2-ME-MAT);and dithiothreitol microagglutination test insert ignore into journalissuearticles values(DTT-MAT);. The sensitivity and specificity of the tests were calculated by using CFT results as the gold standard. A Kappa value was used to measure the agreements between ELISA and the other tests. Sonicated antigen prepared from Brucella melitensis 16M strain,the peroxidase labelled anti-sheep IgG conjugate and ortho-phenylenediamine substrate were used in ELISA. A total of 500 blood samples collected from 36 sheep flocks in Elazığ and environs were examined. One hundred and three insert ignore into journalissuearticles values(20.6%); samples in ELISA, 55 insert ignore into journalissuearticles values(11%);in RBPT, 84 insert ignore into journalissuearticles values(16.8%);in SAT,89 insert ignore into journalissuearticles values(17.8%);in CFT, 79 insert ignore into journalissuearticles values(15.8%);in 2-ME-MAT and 81 insert ignore into journalissuearticles values(16.2%);in DTT- MAT were found to be brucella-positive. In the comparison of these tests,a significant difference was detected between ELISA and RBPT results insert ignore into journalissuearticles values(p0.05);. Excellent agreement was detected between ELISA and CFT, DTT-MAT, 2-ME-MAT and SAT results, with kappa values of 88%, 84%, 83% and 85%, respectively. However,in the comparison of ELISA with RBPT,a kappa value of 64%insert ignore into journalissuearticles values(fair agreement);was obtained. In addition, the sensitivity and specificity of ELISA were determined to be 97%and 96%, respectively.
Keywords : Brucellosis, ELISA, Serological Tests, Sheep, Brucella melitensis