Detection of Leptospira Species by Polymerase Chain Reaction (PCR) in Urine of Cattle

Authors : Burhan ÇETİNKAYA, H Basri ERTAŞ, Hasan ÖNGÖR, Adile MUZ

Pages : 123-130

View : 33 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study was carried out to investigate the prevalance of leptospirosis in the urine of cattle slaughtered in three major abattoirs in the east of Turkey. A polymerase chain reaction insert ignore into journalissuearticles values(PCR); based on a pair of genus-specific primers was used to detect leptospiral DNA in the urine samples of 473 cattle, 284 of which were from Elazig, 112 from Malatya and 77 from Diyarbakir. The detection limit of the method was determined to be approximately five bacteria per ml of urine. In the examination of urine samples, 4.02% insert ignore into journalissuearticles values(19/473); insert ignore into journalissuearticles values(95% confidence intervals [Cl] 2.4-6.2); were found to be positive by PCR. The prevalance of disease was determined to be 3.6% insert ignore into journalissuearticles values(9/250); insert ignore into journalissuearticles values(95% Cl 1.7-6.7); in males and 4.5% insert ignore into journalissuearticles values(10/223); insert ignore into journalissuearticles values(95% Cl 2.2-8.1); in females, but the difference according to sex was not statistically significant insert ignore into journalissuearticles values(P=0.79);. When the prevalance was calculated in different abattoirs, the highest proportion was obtained in Elazig with 4.9% insert ignore into journalissuearticles values(14/284); insert ignore into journalissuearticles values(95% Cl 2.7-8.1);, and the lowest in Malatya with 1.8% insert ignore into journalissuearticles values(2/112); insert ignore into journalissuearticles values(95% Cl 0.2-6.3);. However, the difference between the abattoirs was not significant insert ignore into journalissuearticles values(P=0.35);.
Keywords : Leptospira, cattle, urine, prevalence, PCR