Localization of Haemophilus somnus Antigen by an Immunoper oxidase Technique in Pneumonic Bovine Lungs

Authors : Rıfkı HAZIROĞLU

Pages : 177-180

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :One hundred pneumonic lungs from calves were examined by an immunoper oxidase technique using avidin-biotin- per oxidase complex method in formalin-fixed, paraffin-embedded sections for Haemophilus somnus. In the histological examination of tissue sections, strong positive reactions against H. somnus were detected in 14% of the lungs. H. somnus antigens were observed at the surface and/or within the epithelial cells, macrophages, necrotic alveolar walls, alveolar -bronchial/br onchiolar exudates, fibrins, distended interlobular septa and perivascular areas. In addition, dense antigen accumulations were detected in the necrotic macrophages and neutrophils.
Keywords : Haemophilus somnus, Immunoper oxidase, Pneumoniae