Comparison of Carbohydrate Compositions of Total Apolipoproteins in Lipoproteins

Authors : Tayfun GÜLDÜR, Sema OZAN, Tülay İLERİ

Pages : 777-784

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Terminal carbohydrate moieties of apolipoproteins of lipoproteins in human and goat serum were ascertained and compared. Apolipoproteins of b+pre-b insert ignore into journalissuearticles values(apolipoprotein B containing lipoproteins); and a lipoproteins separated by phosphotungstic acid/MgCl2 precipitation method were applied to SDS-PAGE and blotted onto nitrocellulose membrane. Digoxigenin labelled lectins, each of which recognizes a specific sugar sequence, were incubated with apolipoproteins immobilized on a western blot membrane to probe some of carbohydrate structures commonly found in carbohydrate chains of glycoproteins.The presence of bound lectin on the membrane was detected with alkaline phosphatase labelled antidigoxigenin antibody.Sialic acid terminally linked a insert ignore into journalissuearticles values(2--3); to galactose was detected on the largest protein band in b+pre-b lipoprotein fraction of both human and goat serum with the mobility identical to that of apo B.Neither of the carbohydrate moieties probed were found to be present in a lipoproteins.
Keywords : Lipoprotein, apolipoprotein, carbohydrate moiety