Effects of Maturation and Fertilization Times on Pronuclear Development of Bovine Oocytes Fertilized in Vitro

Authors : Sema BİRLER, Serhat PABUÇÇUOĞLU, Serhat ALKAN, Mithat EVECEN, İ Kamuran İLERİ

Pages : 1-16

View : 25 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Primer oocytes insert ignore into journalissuearticles values(n=520); collected from ovaries of slaughtered cows were divided into 3 groups, and matured at 39ºC with 95-100% humidity and under a gas mixture of 5% O 2 , 5% CO 2 and 90% N 2 for 22, 24 and 26 hours. Modified Parker`s Medium insert ignore into journalissuearticles values(MPM); supplemented with FSH and 20% ECS was used as maturation medium. At the end of these periods, oocytes insert ignore into journalissuearticles values(22 h.=122, 24 h.=143 and 26 h.=255); were taken into Tyrode-lactate medium supplemented with BSA, heparin and PHE, and frozen-thawed bull semen was add after swim-up procedure insert ignore into journalissuearticles values(10µ[email protected] spermatozoa/30-35 oocytes);. Oocytes matured in 3 groups were kept with spermatozoa for 14, 16, 18, 20, 22 and 24 hours. At the end of these time periods all oocytes were fixed, stained and examined by phase-contrast microscope. In total 42 insert ignore into journalissuearticles values(72.4%);, 38 insert ignore into journalissuearticles values(69.1%); and 100 insert ignore into journalissuearticles values(84.7%); oocytes relating to the maturation groups, male and female pronuclei were synchronised. Difference between the 26 h. group and the other groups was highly significant insert ignore into journalissuearticles values(c 2 , p
Keywords : Turk J Vet Anim Sci 1998, 22 1 16 Turk J Vet Anim Sci, vol 22, iss 1