Single nucleotide polymorphism identification and characterization of GnRHR gene in Bos taurus and Bos indicus cattle

Authors : Ramesha KEREKOPPA, Marappa BASAVARAJU, Akhila RAO, Gujjar Ramachandra GEETHA, Kataktalware MUKUND, Jeyakumar SAKTHIVEL

Pages : 10-15

Doi:10.3906/vet-1310-47

View : 13 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The hypothalamic gonadotropin-releasing hormone receptor insert ignore into journalissuearticles values(GnRHR); plays an essential physiological role in reproductive function. In this investigation, we studied genetic variation in the entire coding region of GnRHR gene using PCR-SSCP technique in 250 Bos taurus insert ignore into journalissuearticles values(Holstein Friesian, Jersey); and Bos indicus insert ignore into journalissuearticles values(Malnad Gidda, Deoni); bulls. The genetic variants in GnRHR gene were determined by PCR-SSCP technique by using 5 sets of primers. SSCP analysis of fragment 1, comprising exon 1, and fragment 3, comprising exon 2, revealed 2 SSCP patterns in all breeds under study, while fragment 2 of exon 1 and fragment 4, comprising exon 3, revealed 3 patterns in Malnad Gidda; Holstein Friesian and Deoni breeds showed 2 SSCP patterns. In Jersey bulls, fragment 4 showed 3 patterns. The fragment 5 comprising exon 3 was monomorphic in all 4 breeds under study. SNPs were confirmed by direct sequencing. The sequence analysis revealed 7 transitions insert ignore into journalissuearticles values(T940C, T1021C, T1090C, T1174C, T13940C, T18023C, and G17887A);, 3 transversions insert ignore into journalissuearticles values(G13817C, G17926C, and G17926C);, 2 deletions insert ignore into journalissuearticles values(854DA and 17684DT);, and 2 insertions insert ignore into journalissuearticles values(1009IA and 180100IC); in GnRHR gene. The study revealed high genetic variation in GnRHR gene in cattle.
Keywords : GnRHR, PCR SSCP, single nucleotide polymorphism, cattle