Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats

Authors : Alev Gürol BAYRAKTAROĞLU, Özkan ŞİMŞEK, Aytül KÜRÜM, Şevket ARIKAN, Emel ERGÜN

Pages : 57-61

Doi:10.3906/vet-1407-44

View : 22 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study aimed to determine the alpha-naphthyl acetate esterase insert ignore into journalissuearticles values(ANAE); activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats. In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study. Optimum reaction was achieved after a 3 h incubation period at pH 5.8, by ANAE staining. Two types of reaction were observed in ANAE insert ignore into journalissuearticles values(+); T lymphocytes. The first reaction was a dot-like positivity pattern characterized by the presence of large granules, while the other was a granular positivity pattern characterized by the presence of 3 to 5 small granules. B lymphocytes reacted negatively in ANAE staining. The rate of ANAE insert ignore into journalissuearticles values(+); lymphocytes in pregnant, kitten, and adult Angora cats was 62.89 ± 1.29%, 68.37 ± 1.22%, and 77.71 ± 1.63%, respectively. In conclusion, the lowest rate of ANAE-positive lymphocytes was detected in the pregnant cats, depending on the maternal immune tolerance supported by hormonal mechanisms. An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system.
Keywords : ANAE activity, Angora cats, lymphocyte, pregnancy