The effect of short-term lupin (Lupinus angustifolius) feed supplementation on serum steroid hormones, insulin-like growth factor I, and ovarian follicular development and atresia in Merino ewes

Authors : Radoslava VLCKOVA, Jan POSIVAK, İgor VALOCKY, Zuzana KRAVCOVA, Andrea EIBENOVA, Drahomíra SOPKOVA

Pages : 686-692

Doi:10.3906/vet-1404-23

View : 16 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The effects of short-term lupin supplementation on ovulation rate in ewes during oestrous season are well known. In this study, we tested the effects of an 8-day lupin supplementation on folliculogenesis during anoestrus applied over the hormonal treatment of ewes. Fourteen anoestrous Merino ewes were assigned into 2 groups insert ignore into journalissuearticles values(n = 7 for each); in which the control group was fed a basal diet and the experimental group was supplemented with lupin groats insert ignore into journalissuearticles values(500 g per head per day);. Both groups were treated intramuscularly with 12.5 µg/head lecirelinum insert ignore into journalissuearticles values(luteinising hormone releasing hormone super analogue); on the first day of supplementation and 5 days later with 37.5 µg/head cloprostenolum D insert ignore into journalissuearticles values(prostaglandin F2 alpha analogue);. Serum sexual steroid and insulin-like growth factor I insert ignore into journalissuearticles values(IGF-I); concentrations were assessed using radioimmunoassay and enzyme-linked immunosorbent assay methods, respectively. Folliculogenesis was evaluated using a light microscope. Lupin feeding did not affect progesterone or IGF-I, but it significantly decreased the concentration of oestradiol-17beta. Lupin significantly increased the number of follicles of 3-5 mm in diameter and the total rate of atresia. The follicles mainly collapsed and underwent late atresia. The size of healthy and collapsed follicles increased in the lupin-fed group. These results indicated that 8-day lupin supplementation affected follicular development and atresia through an IGF-I/oestradiol feedback system.
Keywords : Lupin feeding, ovarian follicle, atresia, oestradiol 17beta, insulin like growth factor I