Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

Authors : Babak QASEMI PANAHI, Parviz TAJIK, Mansoureh MOVAHEDIN, Gholamali MOGHADDAM, Mohammad Hossein GERANMAYEH

Pages : 693-698

Doi:10.3906/vet-1402-70

View : 19 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Spermatogonial stem cells insert ignore into journalissuearticles values(SSCs); are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 insert ignore into journalissuearticles values(IGF-1);. During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 ng/mL IGF-1 in vitro improves proliferation and postfreezing viability rate of SSCs.
Keywords : Spermatogonial stem cells, proliferation, IGF 1, bovine