Impact of the depletion of high-abundance proteins in blood plasma/serum on the proteome of these media in growing farm animals

Authors : Adam LEPCZYNSKI, Alicja DRATWACHALUPNIK, Agnieszka HEROSIMCZYK

Pages : 490-495

Doi:10.3906/vet-1404-80

View : 17 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves. Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis insert ignore into journalissuearticles values(2DE);. Bioinformatic analysis of the 2D gels showed an increased number of detected spots on each gel after depletion of excess high-abundance proteins in calf plasma insert ignore into journalissuearticles values(+26.46%); and in piglet serum insert ignore into journalissuearticles values(+49.40%);. Relative expression of selected low-abundance protein spots in the blood plasma/serum samples was higher after high-abundance protein depletion. Both media showed decreased expressions of high-abundance protein spot clusters on 2D gels after treatment. The results of this study confirm both the high efficiency of high-abundance protein depletion and the increase in concentrations of low-abundance proteins on 2D gels after treatment. The method of depletion is reproducible and it may be used as an alternative to immunodepletion for preparing blood plasma/serum samples from young, growing animals for 2DE. Increased content of the low-abundance proteins on the 2D gels enables complete analysis of plasma/serum proteome and thereby allows for the discovery of novel biomarkers that might be useful in veterinary medicine.
Keywords : Proteome, high abundance protein depletion, 2 dimensional electrophoresis, farm animals