Phylogenetic analysis of NS gene of avian influenza viruses (H9N2) isolated from chicken in Iran during 2007-2011

Authors : Hassan NOROUZIAN, Hessam EMADI CHASHMI, Ziba NAZARI, Mehdi VASFI MARANDI

Pages : 501-513

Doi:10.3906/vet-1311-11

View : 13 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :To determine sequence characteristics and phylogenetic relationships of the nonstructural insert ignore into journalissuearticles values(NS); gene, the NS genes of 9 avian influenza insert ignore into journalissuearticles values(AI); viruses were sequenced and compared with other Iranian AI H9N2 viruses available in GenBank. The similarity among the earliest Iranian H9N2 isolate insert ignore into journalissuearticles values(A/chicken/Iran/ZMT-101/1998); and recent insert ignore into journalissuearticles values(2007–2011); studied isolates was low insert ignore into journalissuearticles values(94%);. Some of the studied viruses revealed substitutions in RNA-binding and effector domains as well as antigenic regions of the NS protein. Recently studied isolates have a high similarity insert ignore into journalissuearticles values(97.4%-98.7%); with some Pakistani isolates insert ignore into journalissuearticles values(like A/chicken/Sawabi/NARC-2434/2006); in the NS gene. In the phylogenetic analysis, recently studied isolates composed a cluster separate from other Iranian isolates and were placed in the neighborhood of some Pakistani isolates. Hence, new Iranian H9N2 isolates may originate from Pakistani isolates. The NS protein of the Iranian H9N2 isolates differed from that of the Iranian highly pathogenic H5N1 viruses. The potential risk of the emergence of a pandemic virus because of the reassortment of the internal genes between H9N2 viruses and highly pathogenic AI viruses on one hand and the genetic and amino acid changes of AI viruses affecting their pathogenicity and immunogenicity on the other hand confirm the importance of continued surveillance of AI viruses.
Keywords : Avian influenza, Iran, nonstructural gene, phylogenetic analysis