Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds

Authors : Onur YILMAZ, Tamer SEZENLER, Nezih ATA, Yalçın YAMAN, İbrahim CEMAL, Orhan KARACA

Pages : 354-357

Doi:10.3906/vet-1401-13

View : 17 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The calpastatin insert ignore into journalissuearticles values(CAST); gene has a major effect on muscle growth and meat tenderness after slaughter; it is located on the fifth chromosome in sheep. Blood samples were collected from 720 animals in total from Kıvırcık insert ignore into journalissuearticles values(KIV);, Sakız insert ignore into journalissuearticles values(SZ);, Karacabey Merino insert ignore into journalissuearticles values(KM);, and Gökçeada insert ignore into journalissuearticles values(GA); sheep populations raised in West Anatolia. The PCR products were digested by the restriction endonuclease MspI. Allele frequencies for M and N alleles of the gene were found to be 0.85 and 0.15 in KIV, 0.80 and 0.20 in KM, 0.99 and 0.01 in GA, and 0.34 and 0.66 in SZ sheep, respectively. It was determined that NN genotype frequency was quite lower in KIV insert ignore into journalissuearticles values(0.04); and KM insert ignore into journalissuearticles values(0.07); populations than in the SZ breed insert ignore into journalissuearticles values(0.40);. On the other hand, the NN genotype was not observed in the GA population. Populations other than KM were found to be in Hardy–Weinberg equilibrium. In general, allele and genotype frequencies of CAST were similar to those in other studies on different sheep populations. This research is a beginning step for finding candidate genes’ effects on meat quantity and quality.
Keywords : Calpastatin, sheep, PCR RFLP, MspI