Activity of 3β-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin

Authors : Özkan ŞİMŞEK, Monika MIHM

Pages : 358-362

Doi:10.3906/vet-1311-57

View : 10 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Three-b-hydroxysteroid dehydrogenase insert ignore into journalissuearticles values(3b-HSD); is the enzyme responsible for progesterone production. This study aimed to determine whether 3b-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone insert ignore into journalissuearticles values(FSH);, luteinising hormone insert ignore into journalissuearticles values(LH);, and insulin-like growth factor I insert ignore into journalissuearticles values(IGF-I); concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum insert ignore into journalissuearticles values(FCS);. Subsequently, cells were exposed to FSH insert ignore into journalissuearticles values(1 ng/mL);, LH insert ignore into journalissuearticles values(10 or 100 ng/mL);, or FSH insert ignore into journalissuearticles values(1 ng/mL); + IGF-I insert ignore into journalissuearticles values(1 or 10 ng/mL); in a serum-free medium for another 96 h to predict degree of luteinisation. Before and after incubation, granulosa cells were stained for 3b-HSD activity. The high dose of IGF-I insert ignore into journalissuearticles values(10 ng/mL); increased insert ignore into journalissuearticles values(P < 0.05); progesterone secretion over 2.5-fold compared with FSH alone or the low dose of IGF-I insert ignore into journalissuearticles values(1 ng/mL); in cells preincubated with FCS. This was clearly reflected by darker 3b-HSD staining than in cells exposed to FSH and low dose IGF-I.
Keywords : 3b Hydroxysteroid dehydrogenase, bovine granulosa cells, progesterone, FSH, LH, IGF I