Serological and molecular diagnosis of paratuberculosis in dairy cattle

Authors : Kadir Semih GÜMÜŞSOY, Tuba İÇA, Seçil ABAY, Fuat AYDIN, Harun HIZLISOY

Pages : 147-153

Doi:10.3906/vet-1410-96

View : 20 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, the presence of paratuberculosis was investigated by serological and molecular methods in herds of dairy cattle. Blood, milk, and stool samples of 147 cows aged 2 years old or older with chronic diarrhea were collected. A California mastitis test insert ignore into journalissuearticles values(CMT); was performed on milk samples. Indirect paratuberculosis enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA); test was used for serological investigation. Polymerase chain reaction insert ignore into journalissuearticles values(PCR); was utilized for molecular identification of Mycobacterium avium subsp. paratuberculosis insert ignore into journalissuearticles values(MAP); from milk and stool samples. Immunomagnetic separation insert ignore into journalissuearticles values(IMS); method was used for milk and fecal samples. Eighteen insert ignore into journalissuearticles values(12.24%);, 44 insert ignore into journalissuearticles values(29.93%);, 36 insert ignore into journalissuearticles values(24.49%);, 28 insert ignore into journalissuearticles values(19.05%);, and 21 insert ignore into journalissuearticles values(14.29%); of 147 milk samples were negative, suspicious, CMT insert ignore into journalissuearticles values(+);, CMT insert ignore into journalissuearticles values(++);, and CMT insert ignore into journalissuearticles values(+++); by CMT, respectively. According to ELISA results, 18 insert ignore into journalissuearticles values(12.24%); serum samples were positive. In PCR of milk and stool samples, MAP DNA was detected in 20 insert ignore into journalissuearticles values(13.61%); and 42 insert ignore into journalissuearticles values(28.57%); of samples, respectively. In IMS PCR assays of the same samples, positivity was not detected. In this study, paratuberculosis was found at high rates in Kayseri. In conclusion, it was detected that mastitis symptoms in paratuberculosis were subclinical and not always observed, and the use of diagnostic laboratory methods may be an important aid in revealing diseases.
Keywords : Cattle, enzyme linked immunosorbent assay, immunomagnetic separation, paratuberculosis, polymerase chain reaction