The effect of oleic and linoleic acids on in vitro bovine embryonic development and embryo quality

Authors : Tahir KARAŞAHİN, Şevket ARIKAN

Pages : 154-159

Doi:10.3906/vet-1404-18

View : 14 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid insert ignore into journalissuearticles values(10, 100, and 1000 µM);. Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates insert ignore into journalissuearticles values(P < 0.01);. It was determined that the highest dose of linoleic acid insert ignore into journalissuearticles values(1000 µM); and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morula-blastocyst stage insert ignore into journalissuearticles values(P < 0.001);. As a result, while oleic acid significantly increased insert ignore into journalissuearticles values(P < 0.001); the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.
Keywords : Oleic acid, linoleic acid, bovine embryo, blastocyst