Heparin-induced in vitro capacitation changes of swamp buffalo spermatozoa

Authors : Dibyajyoti TALUKDAR, Kutubuddin AHMED, Sourabh DEORI, Gopal Chandra DAS

Pages : 629-633

View : 17 | Download : 11

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to evaluate heparin-induced in vitro capacitation-associated changes in spermatozoa of swamp buffalo. Therefore, freshly ejaculated and washed spermatozoa of 8 swamp buffalo bulls were capacitated in vitro in TALP medium supplemented with BSA, heparin, and HEPES buffer at a concentration of 6 × 109 spermatozoa/mL at 37 °C for 6 h. Capacitation status of spermatozoa in terms of the hyperactivated motility, acrosome membrane integrity, total hypoosmotic swelling test insert ignore into journalissuearticles values(HOST);, activity of alanine aminotransferase insert ignore into journalissuearticles values(ALT); and aspartate aminotransferase insert ignore into journalissuearticles values(AST);, and sperm membrane protein insert ignore into journalissuearticles values(SMP); and cholesterol content were estimated for each ejaculate at 1-h intervals from 0 to 6 h of incubation. The results revealed that the highest hyperactivation of spermatozoa insert ignore into journalissuearticles values(74.50 ± 1.78%); and live acrosome reaction insert ignore into journalissuearticles values(56.92 ± 1.88%); was recorded at 4 h of incubation while the total HOST-reacted spermatozoa and SMP and cholesterol levels decreased significantly insert ignore into journalissuearticles values(P < 0.01); with increasing period of incubation. The AST and ALT activities increased significantly insert ignore into journalissuearticles values(P < 0.01); as incubation period increased. In conclusion, heparin induces in vitro capacitation changes in swamp buffalo spermatozoa as evidenced by the highest hyperactivation of spermatozoa and live acrosome reaction at 4 h of incubation.
Keywords : Swamp buffalo, spermatozoa, capacitation, alanine aminotransferase, aspartate aminotransferase, protein, cholesterol