Comparison of the in vitro anticancer effect of habanero pepper extract containing capsaicin with that of pure capsaicin in selected dog neoplastic cell lines

Authors : łukasz ADASZEK, Olga SŁABCZYŃSKA, Paweł ŁYP, Dorota RÓŻAŃSKA, Jerzy ZIĘTEK, Maciej ORZELSKI, Anna ŚMIECH, Marta STANIEC, Dorota KRASUCKA, Stanisław WINIARCZYK

Pages : 243-250

View : 19 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this article was to assess the anticancer effectiveness of habanero pepper extract containing capsaicin on two dog cancer lines, DAN insert ignore into journalissuearticles values(fibroblasts isolated from osteosarcoma); and D-17 insert ignore into journalissuearticles values(epithelium cells of osteosarcoma obtained from pulmonary metastatic tumors);, under in vitro conditions. The experiment was carried out in a suspension of D-17 and DAN line cells with a density of 12 × 105 cells/mL in a culture fluid with 10% of FBS. After 24 h of incubation insert ignore into journalissuearticles values(37 °C, 5% CO2);, the fluid above the culture was removed, and diluted habanero pepper extract was added. The concentrations of capsaicin in the extract were 10, 20, 50, 100, 150, and 200 ?M insert ignore into journalissuearticles values(in the culture fluid with 10% of FBS);. The cells were incubated for 24?96 h insert ignore into journalissuearticles values(37 °C, 5% CO2);. After the incubation period, cytotoxicity assessment of the habanero pepper extract and cell proliferation impairment by the habanero pepper extract in a suspension of cell lines, in MTT test, as well as the cytometric examination of viability of cancer cells exposed to the habanero pepper extract were performed. The results indicate that the extract shows better anticarcinogenic activity in vitro compared with pure capsaicin. Confirmation of the anticancer effectiveness of the extract on the cells is a starting point for wide clinical observations and a good indication for further research.
Keywords : Capsaicin, habanero pepper, neoplasma, in vitro study