Evidence of foot-and-mouth disease virus excretion in the milk of apparently healthy vaccinated buffaloes in Islamabad, Pakistan

Authors : Hafiz İftikhar AHMED, Umer FAROOQ, Aamer Bin ZAHUR, Khalid NAEEM, Asma LATIF, Hamid IRSHAD

Pages : 431-434

View : 18 | Download : 14

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of the study was to determine the seroprevalence of foot-and-mouth disease insert ignore into journalissuearticles values(FMD); and isolation of the FMD virus insert ignore into journalissuearticles values(FMDV); in the milk of apparently healthy Asian buffaloes. A cross-sectional study was conducted in periurban dairy farms insert ignore into journalissuearticles values(n = 20); on ≥20 animals per farm in the suburbs of Islamabad, Pakistan. Sera samples insert ignore into journalissuearticles values(n = 200); were collected to monitor FMDV seroconversions. The analysis of serum samples using ELISA indicated a high seroprevalence of FMD insert ignore into journalissuearticles values(78%; 156/200); in Asian buffaloes. Milk samples were collected from FMDV seropositive animals only, and virus-specific signals were detected in 12.2% insert ignore into journalissuearticles values(19/156); of the animals by reverse transcriptase real-time PCR insert ignore into journalissuearticles values(rRT-PCR);. Milk samples positive for FMDV using rRT-PCR were inoculated into LF-BK cells for virus isolation. Two FMDV isolates insert ignore into journalissuearticles values(serotypes O and Asia-1); were recovered from the milk samples. A high seroprevalence of FMD and isolation of FMDV from the milk of healthy Asian buffaloes may represent a possible role for healthy Asian buffaloes as an FMD reservoir.
Keywords : FMD, seroprevalence, ELISA, milk, buffalo, LF BK, real time PCR