Evaluation of diagnostic methods for the detection of pestiviruses in clinical samples

Authors : Pelin TUNCER GÖKTUNA, Kadir YEŞİLBAĞ

Pages : 175-179

View : 27 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :There are several commonly used diagnostic methods to detect pestiviruses for routine diagnostic purposes. In the present study, we aimed to compare virus isolation-indirect immunoperoxidase monolayer assay insert ignore into journalissuearticles values(VI-IIPMA);, antigen capture ELISA insert ignore into journalissuearticles values(ACE);, and RT-PCR for the detection of pestiviruses in clinical samples. Out of 246 samples tested insert ignore into journalissuearticles values(11 serum, 119 swab, 116 tissue);, 28 samples insert ignore into journalissuearticles values(11.39%); were positive and 218 insert ignore into journalissuearticles values(88.61%); were negative using the VI-IIPMA method. Using ACE, 70 samples insert ignore into journalissuearticles values(28.46%); were positive and 176 insert ignore into journalissuearticles values(71.54%); were negative. Finally, using RT-PCR analysis, we detected 19 insert ignore into journalissuearticles values(7.72%); positive and 227 insert ignore into journalissuearticles values(92.28%); negative samples. Inconsistencies were observed among results of the three methods: 8 samples were positive using VI-IIPMA but negative using ACE and RT-PCR. In addition, 4 samples that were found to be negative by VI-IIPMA were found to be positive by ACE and RT-PCR. Five samples were positive by ACE and VI-IIPMA. However, 46 samples were found to be positive only by ACE. These results show that the number of positive results detected by ACE is higher than that by VI-IIPMA and RT-PCR. Although ACE may prove advantageous for diagnosing pestiviruses, using a second method in combination with ACE will improve the validity of the results.
Keywords : Pestiviruses, ELISA, immunoperoxidase monolayer assay, virus isolation, RT PCR