Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

Authors : Nilgün ÜNAL, Alper KARAGÖZ, Şinasi AŞKAR, Zahide DİLİK, Buket YURTERİ

Pages : 72-76

View : 16 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase insert ignore into journalissuearticles values(ESBL);-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit insert ignore into journalissuearticles values(Becton Dickinson, USA); and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis insert ignore into journalissuearticles values(PFGE); was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic ESBL-producing E. coli isolates were identified, eight of them had only the blaTEM-1. Twenty-five ESBL-producing isolates were detected. This research is the first on the investigation and detection of ESBL-producing E. coli isolates from broilers in Turkey. In this study, 8.3% ESBL-producing E. coli were isolated from the cloacal samples of broilers collected from slaughterhouses in Turkey. CTX-M-15 insert ignore into journalissuearticles values(80%); was the most frequently isolated ESBL type. Using PFGE analysis, it was determined that these isolates had clonal similarity.
Keywords : Antimicrobial resistance, broilers, Escherichia coli, extended spectrum beta lactamase, pulsed field gel electrophoresis, CTX M 15