Phylogenetic relationships of native Turkish cattle breeds using microsatellite markers

Authors : Yusuf ÖZŞENSOY, Ercan KURAR, Müge DOĞAN, Zafer BULUT, Mehmet NİZAMLIOĞLU, Ayşe IŞIK, Aysun ÇAMLIDAĞ, Vahdettin ALTUNOK

Pages : 23-29

View : 18 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey insert ignore into journalissuearticles values(AG);, Anatolian Black insert ignore into journalissuearticles values(AB);, South Anatolian Red insert ignore into journalissuearticles values(SAR);, East Anatolian Red insert ignore into journalissuearticles values(EAR);, Southern Anatolian Yellow insert ignore into journalissuearticles values(SAY);, and Zavot insert ignore into journalissuearticles values(ZAV);. DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest insert ignore into journalissuearticles values(7.80); and highest insert ignore into journalissuearticles values(10.80); mean allele numbers were observed for the ZAV and SAY populations, respectively. TGLA122 was the most polymorphic locus; however, only 7 different alleles were observed for INRA005. A total of 40 different private alleles were determined. The general FIS values were between 0.034 and 0.123. Due to the close location to the domestication center, higher genetic diversities were observed. The observed genetic diversities and the results of the phylogenetic analyses were in agreement with evolutionary history and the geographical origins of Turkish native cattle breeds.
Keywords : Cattle, Anatolia, genetic diversity, microsatellite, TÜRKHAYGEN 1