Chemical, enzymatic, and antioxidant enrichments of full-fat soybean and sunflower meal by Bacillus subtilis fermentation using a solid-state bioreactor

Authors : YAŞAR KARAKURT, Damla GÜVERCİN, SERCAN ÖNDER, CİVAN ÇELİK, Ramazan TOSUN, BURAK BARAN, Sulhattin YAŞAR

Pages : 82-93

View : 23 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study was conducted to fortify nutritional and chemical composition of sunflower meal insert ignore into journalissuearticles values(SFM); and full-fat soybean insert ignore into journalissuearticles values(FFSB); used as a protein source in farm animal nutrition by Bacillus subtilis ATCC 6633 fermentation. An optimized set of fermentation parameters insert ignore into journalissuearticles values(60% moisture, 6.5-7.0 pH, 30-35 °C temperature, continuous aeration of 0.5-1.0 L/min and agitation of 20-100 rpm); were used to ferment SFM and FFSB samples with or without using bacterial inoculant for 0, 24, 48, and 72 h. The results indicated that the total organic acids insert ignore into journalissuearticles values(TOA); contents and crude ash insert ignore into journalissuearticles values(CA); of SFM and FFSB increased significantly, but there were significant reductions in dietary fiber insert ignore into journalissuearticles values(DF); components insert ignore into journalissuearticles values(acid detergent fiber insert ignore into journalissuearticles values(ADF); and neutral detergent fiber insert ignore into journalissuearticles values(NDF);); in both feed samples. Moreover, urease activity, trypsin inhibitors insert ignore into journalissuearticles values(TI);, and phytic acid insert ignore into journalissuearticles values(PA); contents of both feed samples reduced by 50%, 35%, and 79%, respectively. Total flavonoid level reduced by 30% in FFSB samples, but remained unchanged in SFM samples. The levels of tannin in FFSB and SFM decreased significantly. Although there were no significant changes in the activities of amylase, celulase, and beta-glucanase; the protease and phytase activities increased significantly. The total phenolic compounds content and the antioxidant activities of FFSB and SFM samples increased significantly. In conclusion, the fermented FFSB insert ignore into journalissuearticles values(F-FFSB); and fermented SFM insert ignore into journalissuearticles values(FSFM); had lowered the levels of ADF, NDF, tannin, TI, urease activity, and PA but they were remarkably enriched with organic acids, enzymes, and antioxidants. These feed materials could be used as functional feed additives or feed materials in farm animal nutrition.
Keywords : Bacillus subtilis, nutrient and chemical enrichment, fermentation, full fat soybean, sunflower meal