Phylogrouping and antimicrobial resistance analysis of extraintestinal pathogenic Escherichia coli isolated from poultry species

Authors : Sujata BHAVE, Rahul KOLHE, RANJAN MAHADEVASWAMY, CHANDRAKANT BHONG, SAMIR JADHAV, Saba NALBAND, DATTATRAY GANDHALE, DUSHYANT MUGLIKAR

Pages : 117-126

View : 17 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study was undertaken to determine the prevalence and antimicrobial resistance pattern of extraintestinal pathogenic Escherichia coli insert ignore into journalissuearticles values(ExPEC); in the feces of healthy poultry and retail chicken. All 146 E. coli recovered from 351 samples were screened by PCR for detection of ExPEC strains. Nineteen insert ignore into journalissuearticles values(13.01%); isolates were confirmed as ExPEC. Distribution of ExPEC strains was revealed as follows: broiler insert ignore into journalissuearticles values(25%);, layer insert ignore into journalissuearticles values(15.87%);, and raw chicken insert ignore into journalissuearticles values(3.12%);. Turkey, duck, and water samples were negative for ExPEC strains. ExPEC strains belonged to phylogenetic groups B2 insert ignore into journalissuearticles values(52.63%);, A insert ignore into journalissuearticles values(36.84%);, and D insert ignore into journalissuearticles values(10.53%);. Twenty-three insert ignore into journalissuearticles values(15.75%); isolates were ESBL-positive, including four ExPEC strains. ESBL-positive E. coli were isolated from all the samples except turkey. A high degree of resistance to commonly used antimicrobials, namely nalidixic acid insert ignore into journalissuearticles values(95.89%);, tetracycline insert ignore into journalissuearticles values(95.89%);, trimethoprim insert ignore into journalissuearticles values(89.04%);, colistin insert ignore into journalissuearticles values(82.88%);, and ciprofloxacin insert ignore into journalissuearticles values(54.11%);, including ß-lactam antimicrobials ampicillin insert ignore into journalissuearticles values(84.93%); and amoxicillin/clavulanic acid insert ignore into journalissuearticles values(81.51%);, was expressed by the isolates. Out of fifteen randomly selected ESBL-positive E. coli isolates, ß-lactam genes, namely blaTEM, blaCTX-M, and blaOXA, were detected in three, six, and one, respectively. Poultry and raw chicken harbor multidrug-resistant, ESBL-type E. coli as well as ExPEC and these strains may be transmitted to humans via the food chain.
Keywords : Extraintestinal pathogenic E coli, ESBL, antimicrobial resistance, poultry