Comparative evaluation of factors affecting hemagglutinatingactivity of avian influenza (H9) virus

Authors : Asfa RASOOL, Sameera AKHTAR, Khushi MUHAMMAD, Masood RABBANI, Aftab Ahmed ANJUM, Javed MUHAMMAD, Arfan AHMAD, Ali Ahmad SHEIKH, Fakhra LIAQAT, Fariha AKHTAR, Rabia TAHIR

Pages : 102-106

View : 22 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Influenza virus H9 was standardized for hemagglutination assay using different factors such as red blood cell insert ignore into journalissuearticles values(RBC); types, concentrations, diluent types, and storage times. Avian influenza virus H9 was grown in embryonated chicken eggs and confirmed by spot agglutination. A significant insert ignore into journalissuearticles values(P < 0.05); difference with the highest mean titer insert ignore into journalissuearticles values(9.00 ± 0.00); was observed using RBCs from different species. Nonsignificant differences insert ignore into journalissuearticles values(P > 0.05); were found between human blood type O, chicken, and dog RBCs, as well as among rabbit, pigeon, sheep, and parrot. The highest titer insert ignore into journalissuearticles values(9.00 ± 0.00); with a nonsignificant difference was found using virus stored at 4 °C and -20 °C while 37 °C showed the lowest significant mean hemagglutinin insert ignore into journalissuearticles values(HA); titer insert ignore into journalissuearticles values(11.08 ± 188.21);. Nonsignificant differences were observed in HA titers against H9 virus stored for 3, 4, 5, and 6 months. Nonsignificant differences were found between the use of normal saline and 0.5% peptone water with the lowest HA titers of 7.83 ± 0.40 and 8.00 ± 0.00, respectively, while the highest HA titer insert ignore into journalissuearticles values(9.00 ± 0.00); with nonsignificant difference was observed by using HA-HI buffer and phosphate buffer saline as diluents. RBCs with 0.5% and 1% concentrations showed nonsignificant difference in HA titer but significant difference with 0.1% RBCs.
Keywords : Avian influenza, hemagglutination, chicken red blood cells, diluents, temperature