Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate

Authors : Pinar Peker AKALIN, Nuri BAŞPINAR, Kenan ÇOYAN, Mustafa Numan BUCAK, Şükrü GÜNGÖR, Caner ÖZTÜRK

Pages : 195-199

View : 21 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate insert ignore into journalissuearticles values(%);, mitochondrial activity rate insert ignore into journalissuearticles values(%);, levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams insert ignore into journalissuearticles values(n = 3); and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased insert ignore into journalissuearticles values(P < 0.05); while mitochondrial activity rates decreased insert ignore into journalissuearticles values(P < 0.05);. In relation with sonication duration time, total antioxidant potential levels increased insert ignore into journalissuearticles values(P < 0.05); in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.
Keywords : Sonication, spermatozoa, damaged sperm rate, mitochondrial activity, lipid peroxidation