Cryopreservation of seabream (Sparus aurata) semen and fertility

Authors : Mehmet Borga TIRPAN, Erkut Derviş ÖZGÖRAY, Havva ALEMDAR, Ergün AKÇAY

Pages : 200-206

View : 19 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, the aim was to reveal spermatological parameters of seabream insert ignore into journalissuearticles values(Sparus aurata);, freezing ability of semen in different extenders, and fertilization capacity of frozen seabream semen. The study was conducted with 10 male and 6 female brooder seabreams. Female fish were synchronized by photoperiod and water temperature in the breeding season. Four different extenders [Mounib, Salty Mounib, Ringer, and sea water insert ignore into journalissuearticles values(1% NaCl);] including 10% dimethyl sulfoxide were used to assess the freezing ability of seabream semen. After postthaw spermatological examinations, three successful extenders insert ignore into journalissuearticles values(Mounib, Ringer, 1% NaCl); were chosen for the fertilization stage and 2 control and 3 experimental groups were formed insert ignore into journalissuearticles values(control I: fresh semen, control II: naturally fertilized eggs);. When postthaw motility values were considered, the best results belonged to Mounib and Ringer extenders at 54.45 ± 1.18% and 58.25 ± 1.97%, respectively. At the fertilization stage, the highest hatching rates were from Mounib and Ringer at 29.20 ± 1.05% and 28.63 ± 1.12%, respectively. The statistical difference between extenders was significant insert ignore into journalissuearticles values(P < 0.05);. Mounib and Ringer extenders gave the highest motility and fertility rates. As a result, Mounib and Ringer extenders can be used for freezing seabream semen, and semen frozen with these extenders supplies sufficient fertility results.
Keywords : Seabream semen extender, Sparus aurata, semen cryopreservation, Turkey, fertility