Mycoflora and chemotype characterization of Fusarium graminearum isolatesobtained from corn and barley used as feedstuffs in the northeast of Iran

Authors : Abdollah SALEHI, Roya Rezaeian DOLOEI

Pages : 337-343

View : 12 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study aimed to investigate the mycoflora and chemotyping of Fusarium graminearum isolates in corn and barley used in industrial dairy farms in Khorasan-Razavi Province, northeastern Iran. For this purpose, a total of 128 corn and barley samples were collected from 16 different large industrial dairy farms. Mean moisture of the samples collected in different seasons of the year was significantly different insert ignore into journalissuearticles values(P < 0.05);. The most prevalent fungi isolated from corn samples were Penicillium spp. insert ignore into journalissuearticles values(90.62%); and Aspergillus spp. insert ignore into journalissuearticles values(82.81%);, and that of barley samples was Penicillium spp. insert ignore into journalissuearticles values(89.06%);. Frequency of Fusarium spp. İn corn and barley samples was 85.93% and 26.56%, respectively. All isolates insert ignore into journalissuearticles values(100%); were confirmed as Fusarium using an ITS primer. Eight insert ignore into journalissuearticles values(11.11%); out of 72 Fusarium spp. İsolates were confirmed as F. Graminearum using species-specific primers. Five out of 8 F. Graminearum isolates were positive in terms of the presence of the trichothecene-producing gene using a Tri5-specific primer. Three and 2 out of 5 toxin-producing F. Graminearum isolates were positive using Tri13DON- and Tri13NIV-specific primers, respectively. In conclusion, the presence of toxigenic Fusarium in corn and barley samples used in feedstuffs in Iran is a potential risk to animal and human health.
Keywords : Fusarium graminearum, deoxynivalenol, nivalenol, chemotype, polymerase chain reaction