Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Authors : Aytül KÜRÜM, Siyami KARAHAN, Hakan KOCAMIŞ, Miyase ÇINAR, Emel ERGÜN

Pages : 448-455

View : 23 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase insert ignore into journalissuearticles values(GPX-I);, superoxide dismutase insert ignore into journalissuearticles values(SOD);, and catalase insert ignore into journalissuearticles values(CAT); in bovine oviduct epithelial cells insert ignore into journalissuearticles values(BOEC); isolated from the isthmus and ampulla of the oviduct at estral insert ignore into journalissuearticles values(n = 7); and luteal phases insert ignore into journalissuearticles values(n = 7); of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages in both sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases with a significant difference for the estral phase insert ignore into journalissuearticles values(P < 0.05);, and it decreased over the passages with no significant differences. In conclusion, the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higher in the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitro environment.
Keywords : Ampulla, bovine oviduct epithelial cell, catalase, glutathione peroxidase, isthmus, superoxide dismutase