Serodiagnostic potential of Brucella outer membrane and periplasmic proteins

Authors : Aitbay BULASHEV, Orken AKIBEKOV, Zhanbolat SURANSHIYEV, Bakytkali INGIRBAY, Saule ESKENDIROVA

Pages : 486-493

View : 12 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to evaluate the serological diagnostic potential of the Brucella recombinant outer membrane insert ignore into journalissuearticles values(rOMP25, rOMP31); and periplasmic proteins insert ignore into journalissuearticles values(rBP26, rSOD); in a comparative way using an indirect enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(i-ELISA);. Rabbit and/or mouse antibodies to Brucella whole cell and/or soluble protein preparations recognized all recombinant proteins used, which confirms the expression of target antigens in E. coli in active form. The recombinant proteins showed different antigenicity to antibodies of cattle kept on a brucellosis-affected insert ignore into journalissuearticles values(endemic); farm and/or a new focus of infection. Thus, the presence of anti-Brucella antibodies was confirmed by i-ELISA/rSOD in 79% of cows from endemic conditions with positive results by conventional serological tests insert ignore into journalissuearticles values(RBPT and/or CFT);. However, antibodies specific to this protein were detected in only 14% of seropositive animals kept in the hotbed of a new brucellosis infection. Moreover, rSOD-specific antibodies were not detected in the sera of vaccinated cattle from a brucellosis-free farm, whereas antibodies to other recombinant proteins were found in 2%-8% of animals. Using recombinant proteins in immunoassays significantly reduced the number of cows positive for brucellosis. Furthermore, there was not a single protein among the rOMPs that would show the total positive results of all proteins used. Thus, the development of reliable ELISA tests for the diagnosis of brucellosis requires further comprehensive study of the recombinant proteins in order to design a multiprotein antigen that consists of a combination of several proteins with diagnostic potential.
Keywords : Brucellosis, cattle, diagnosis, recombinant proteins, enzyme linked immunosorbent assay