Effect of various antioxidants and their combinations on bull semen cryopreservation

Authors : Şükrü GÜNGÖR, Ayhan ATA, Muhammed Enes İNANÇ, John P KASTELIC

Pages : 590-595

View : 24 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Our objective was to evaluate the effects of adding 50 mM trehalose insert ignore into journalissuearticles values(TRE);, 1 mM cysteine insert ignore into journalissuearticles values(CYS);, or 400 µg catalase insert ignore into journalissuearticles values(CAT);, individually or in combinations, to a Tris-based semen extender and determine the effects on fresh, chilled-equilibrated, and frozen-thawed bull sperm. Ejaculates from three Holstein bulls insert ignore into journalissuearticles values(1-3 years old); were pooled, divided into six equal portions, and put into a Tris-based extender supplemented with no additive insert ignore into journalissuearticles values(control);, TRE, CYS, CAT, TRE + CYS, or TRE + CYS + CAT. In chilled-equilibrated semen, motility was highest in CAT but lowest in the control insert ignore into journalissuearticles values(P < 0.05);. In thawed semen, motility was lower insert ignore into journalissuearticles values(P < 0.05); in CAT than in all other groups, which were not different. Based on a hypoosmotic swelling test insert ignore into journalissuearticles values(HOS test);, TRE, CYS, and their combinations had higher insert ignore into journalissuearticles values(P < 0.05); percentages of sperm with an intact plasma membrane than the control or CAT groups. The TRE group had the least insert ignore into journalissuearticles values(P < 0.05); morphologically abnormal sperm. Plasma membrane and acrosomal integrity insert ignore into journalissuearticles values(PMAI); and high mitochondrial membrane potential insert ignore into journalissuearticles values(HMMP); values were highest in TRE and TRE + CYS and were lower insert ignore into journalissuearticles values(P < 0.05); in the control. Furthermore, HMMP was positively correlated with PMAI and HOS tests, but negatively correlated with major + minor abnormalities insert ignore into journalissuearticles values(r = 0.66, 0.242, and -0.349);. In conclusion, the addition of TRE, with or without CYS, to a Tris-based semen extender improved several aspects of the frozen-thawed quality of bull semen. However, addition of CAT, by itself or in combination with other additives, did not protect bull sperm against cryopreservation-induced defects.
Keywords : Antioxidant, bull semen, flow cytometry, spermatological characteristics, trehalose