Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis

Authors : Teoman KANKILIÇ, Canan GÜRPINAR

Pages : 67-78

Doi:10.3906/sag-1307-147

View : 16 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Genetic differences in 2 regions insert ignore into journalissuearticles values(D-loop and cytochrome b); of mitochondrial DNA in Nannospalax xanthodon and Nannospalax ehrenbergi were determined using restriction fragment length polymorphism insert ignore into journalissuearticles values(RFLP); analysis. For 2 species distributed in Anatolia, 94 specimens from 62 populations of Nannospalax xanthodon and 2 populations of Nannospalax ehrenbergi were studied. To reveal genetic differences, the D-loop insert ignore into journalissuearticles values(626 bp); and cytochrome b insert ignore into journalissuearticles values(500 bp); regions of mitochondrial DNA were amplified and then cut by 4 different restriction endonuclease enzymes insert ignore into journalissuearticles values(BamH-I, Taq-I, Alu-I, and Msp-I);. RFLP analysis revealed 4 haplotypes for the D-loop region and 8 haplotypes for the cytochrome b region in 94 specimens. According to the results of this study, N. nehringi, which is distributed in eastern Anatolia, is a valid species and not a synonym of N. xanthodon. A total of 2 additional species insert ignore into journalissuearticles values(N. nehringi and N. labaumei); occur with N. xanthodon and N. ehrenbergi; thus, Anatolian blind mole rats cannot be represented by only 2 species.
Keywords : Key words Nannospalax, mtDNA, Turkey