Investigation of the influence of the juvenile hormone analogue fenoxycarb on major hemolymph proteins of the silkworm Bombyx mori during the last larval instar

Authors : Ramazan URANLI, Ebru GÖNCÜ, Osman PARLAK

Pages : 223-236

View : 19 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Fenoxycarb 0-ethyl N-insert ignore into journalissuearticles values(2-insert ignore into journalissuearticles values(4-pheoxyphenoxy);-ethyl); carbamate is the most potent juvenile hormone analogue insert ignore into journalissuearticles values(JHA); against a variety of insect species including the silkworm Bombyx mori. In this study, we aimed to demonstrate the effects of this juvenile hormone on major hemolymph proteins of Bombyx mori during the fifth larval instar, by using fenoxycarb and clarifying the interactions between these proteins and the juvenile hormone. Topical applications of fenoxycarb to the fifth instar larvae of Bombyx mori were performed immediately after the fourth ecdysis insert ignore into journalissuearticles values(on day 0); and at the beginning of the prepupal period insert ignore into journalissuearticles values(on day 6);. Fenoxycarb application on day 0 increased the length of instar in both sexes and induced high total protein content in the hemolymph. When fenoxycarb application was performed on day 6, there was no difference in the length of larval instar and a similar total protein amount was reached with control. Detected hemolymph proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis insert ignore into journalissuearticles values(SDS-PAGE);: storage proteins insert ignore into journalissuearticles values(72 kDa-76 kDa);, 30kDa proteins, lipophorin insert ignore into journalissuearticles values(230 kDa);, and vitellogenins insert ignore into journalissuearticles values(178 kDa and 42 kDa);. Our results suggest that major hemolymph proteins have different sensitivities to fenoxycarb and that application days and sex are more important factors to get response.
Keywords : Key words 30K proteins, Bombyx mori, fenoxycarb, hemolymph proteins, lipophorins, storage proteins, vitellogenins